Generator

Part:BBa_K393000:Design

Designed by: Jon Russel, Leah Itagaki   Group: iGEM10_Yale   (2010-10-25)


phsABC_generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2069
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 121
    Illegal AgeI site found at 2644
    Illegal AgeI site found at 3427
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR amplified off the pSB74 plasmid to make it RFC[10] compatible.


Source

Cut out of the plasmid pSB74 (Engineering Hydrogen Sulfide Production and Cadmium Removal by Expression of the Thiosulfate Reductase Gene (phsABC) from Salmonella enterica Serovar Typhimurium in Escherichia coli; Sang-Weon Bang, Douglas S. Clark, and Jay D. Keasling; Applied and Environmental Microbiology, September 2000, p. 3939-3944, Vol. 66, No. 9.

References